Electrical stimulation of tissues has been found to have many uses in pain management, antibacterial treatment, and wound healing. In vivo, it is known to stimulate epidermal migration and increase fibroblast cell proliferation. Here the effects of electrical field (EF) stimulation on collagen, elastin, and collagenase expression in human dermal fibroblasts are studied. The cells are stimulated in bioreactor using square wave voltage pulses controlled by potentiostat for up to 24 h period. The pulse voltage (0–10V), pulse bias (0, +,−), pulse time (10–1000 ms), rest time (0.1–10 s) was varied. The effects of EF stimulation is evaluated in terms of protein expression level and changes in cell morphology. The results show that the expressions of these proteins are correlated and are doubled when EF stimulation larger than 3V and positive bias is applied. The shorter pulse time stimulates the cells more effectively, while the rest time between pulses has smaller effect.
|Commitee:||Brazier, Christopher, McAbee, Douglas|
|School:||California State University, Long Beach|
|Department:||Chemistry and Biochemistry|
|School Location:||United States -- California|
|Source:||MAI 56/04M(E), Masters Abstracts International|
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