Dissertation/Thesis Abstract

Influence of Microvascular Cells on Stem Cell Derived Spinal Motor Neurons
by Sances, Samuel, Ph.D., Cedars-Sinai Medical Center, 2017, 158; 10231221
Abstract (Summary)

Human ALS patient samples can be converted into motor neurons for the study of ALS pathogenesis in vitro. A major challenge, however is the lack of maturation of the stem cell-derived tissue, representing a major barrier to therapy development. The body of work herein details a series of studies to interrogate the relative maturation of induced pluripotent stem cell derived MNs and determine the effects of brain microvascular endothelial cells on their maturation. Finally, a novel microphysiological system for the modeling of ALS and the blood brain barrier is developed that includes the co-culture of brain microvascular endothelial cells under continuous flow of media with a focus on motor neuron function and vascular infiltration.

Indexing (document details)
Advisor: Tagliati, Michele, Svendsen, Clive
Commitee:
School: Cedars-Sinai Medical Center
Department: Biomedical Science and Translational Medicine
School Location: United States -- California
Source: DAI-B 78/09(E), Dissertation Abstracts International
Source Type: DISSERTATION
Subjects: Medicine
Keywords: Als, Microvascular endothelial cells, Motor neuron function, Pluripotent stem cells, Stem cells, Vascular infiltration
Publication Number: 10231221
ISBN: 978-1-369-75184-0
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