Human ALS patient samples can be converted into motor neurons for the study of ALS pathogenesis in vitro. A major challenge, however is the lack of maturation of the stem cell-derived tissue, representing a major barrier to therapy development. The body of work herein details a series of studies to interrogate the relative maturation of induced pluripotent stem cell derived MNs and determine the effects of brain microvascular endothelial cells on their maturation. Finally, a novel microphysiological system for the modeling of ALS and the blood brain barrier is developed that includes the co-culture of brain microvascular endothelial cells under continuous flow of media with a focus on motor neuron function and vascular infiltration.
|Advisor:||Tagliati, Michele, Svendsen, Clive|
|School:||Cedars-Sinai Medical Center|
|Department:||Biomedical Science and Translational Medicine|
|School Location:||United States -- California|
|Source:||DAI-B 78/09(E), Dissertation Abstracts International|
|Keywords:||Als, Microvascular endothelial cells, Motor neuron function, Pluripotent stem cells, Stem cells, Vascular infiltration|
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