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Dissertation/Thesis Abstract
Human ALS patient samples can be converted into motor neurons for the study of ALS pathogenesis in vitro. A major challenge, however is the lack of maturation of the stem cell-derived tissue, representing a major barrier to therapy development. The body of work herein details a series of studies to interrogate the relative maturation of induced pluripotent stem cell derived MNs and determine the effects of brain microvascular endothelial cells on their maturation. Finally, a novel microphysiological system for the modeling of ALS and the blood brain barrier is developed that includes the co-culture of brain microvascular endothelial cells under continuous flow of media with a focus on motor neuron function and vascular infiltration.
| Advisor: | Tagliati, Michele, Svendsen, Clive |
| Commitee: | |
| School: | Cedars-Sinai Medical Center |
| Department: | Biomedical Science and Translational Medicine |
| School Location: | United States -- California |
| Source: | DAI-B 78/09(E), Dissertation Abstracts International |
| Source Type: | DISSERTATION |
| Subjects: | Medicine |
| Keywords: | Als, Microvascular endothelial cells, Motor neuron function, Pluripotent stem cells, Stem cells, Vascular infiltration |
| Publication Number: | 10231221 |
| ISBN: | 9781369751840 |
