Dissertation/Thesis Abstract

The effect of component base pairs on the stability of Potato Spindle Tuber Viroid (PSTVd) loop E RNA
by Salvo, Renee M., Ph.D., University of the Sciences in Philadelphia, 2017, 168; 10585744
Abstract (Summary)

Viroids are small autonomously replicating plant pathogens consisting of non-encapsidated, circular, single-stranded RNA. Viroid RNA does not code for proteins; therefore their primary function is to interact with host proteins or other RNAs to carry out replication and pathogenesis. The potato spindle tuber viroid (PSTVd) is composed of 359 nucleotides and adopts a rod-like secondary structure. The viroid has a central conserved region that contains a loop E motif. During various stages of viroid replication, the loop E motif takes on different folds, even though the sequence of the monomer remains unchanged. The fold of the loop E sequence dictates the cleavage and ligation that create the final infectious viroid circle. Although the loop E motif of PSTVd has been indicated as an important aspect of viroid replication, no direct evidence of what stabilizes the motif in certain folds of replication has been shown. We show the contribution of component base pairs to stability using susceptibility to chemical probes of both wild-type and mutated loop E sequences.

Indexing (document details)
Advisor: Bruist, Michael
Commitee:
School: University of the Sciences in Philadelphia
School Location: United States -- Pennsylvania
Source: DAI-B 78/08(E), Dissertation Abstracts International
Source Type: DISSERTATION
Subjects: Plant Pathology, Biochemistry
Keywords: Plant pathogens, RNA motifs, Virioids
Publication Number: 10585744
ISBN: 9781369646955
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