Members of the oxytocin/vasopressin superfamily of peptides are found in both vertebrate and invertebrate animals including annelid worms. In the earthworm Eisenia foetida the native peptide annetocin has been shown to regulate the smooth muscle of the crop-gizzard. The goal of this study is to determine whether the pharmacological profile of the native receptor in Lumbricus terrestris parallels that of the one in E. foetida by examining the concentration dependent effects of vertebrate neurotransmitters oxytocin, vasotocin, arginine vasopressin, and lysine vasopressin as well as annetocin on the longitudinal smooth muscles of the crop-gizzard and the circular smooth muscles of gizzard ring. With respect to rate of contraction, our results show that the L. terrestris receptor responds to annetocin with a threshold value of 10 -8 M - 10-7 M and a 10-fold or 100-fold higher potency than oxytocin or vasotocin and the pressins, respectively. In addition, absence of myoactivity from the vasopressin peptides was observed on the crop-gizzard. Pharmacological characterization of the receptor with the OT/VP peptides are displayed with the following continuum in decreasing order of potency with respect to rate of contraction: annetocin > oxytocin > arginine vasotocin = arginine vasopressin = lysine vasopressin.
|Commitee:||Kitz, Dennis, Williams, Jason|
|School:||Southern Illinois University at Edwardsville|
|School Location:||United States -- Illinois|
|Source:||MAI 56/03M(E), Masters Abstracts International|
|Subjects:||Endocrinology, Pharmacology, Zoology|
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