It is known that antibiotics play a role in clearing bacterial infections, and sometimes yeast infections. Antibiotics can either kill or inhibit bacteria in many different ways, but they also effect the role white blood cells play in an infection. Some will enhance white blood cell effects, while other will inhibit. How antibiotics do this is not well understood. Azithromycin and daptomycin are two different antibiotics. Very little is known on how they affect the immune response, including phagocytosis, delayed type hypersensitivity (DTH), and cytokine release. Murine macrophages and neutrophils were treated with azithromycin or daptomycin, along with yeast, Candida lusitaniae, and plated to see the effects of the antibiotic on macrophage and neutrophil phagocytosis. Mice were also sensitized to dinitrofluorobenzene and given azithromycin or daptomycin to see how the antibiotics effect DTH. Lastly, an ELISA was done to see how much of the cytokine, IL-6, the macrophages treated with the antibiotics produced compared to the untreated macrophages. It was seen that both azithromycin and daptomycin have significant effects on phagocytosis and DTH. More research is needed to see the effects of the antibiotics on IL-6 release, as well as their effects on other aspects of the immune response.
|Commitee:||McCracken, Vance, Theodorakis, Christopher|
|School:||Southern Illinois University at Edwardsville|
|School Location:||United States -- Illinois|
|Source:||MAI 56/03M(E), Masters Abstracts International|
|Subjects:||Microbiology, Pharmacology, Immunology|
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