The purpose of this study was to examine the effects of training at varying levels of body weight support on running biomechanics. The 8-week training intervention had volunteers (n=33) complete 24 sessions running at either no support (100BW), 25% body weight support (75BW), or 50% body weight support (50BW). Video footage was captured of the runners’ pre- and post- training at the three levels of BW support and stride kinematics were determined. Differences in stride characteristics and joint kinematics between the three BW support conditions and two time points of data collection were evaluated using a 3 x 2 x 3 mixed ANOVA. The 75BW and 50BW group elongated their stride and quickened their cadence as a result to training when evaluated at 75BW and 50BW. Joint kinematics did not change as a result to training. Gait was not altered when returning to 100BW after training at 75BW or 100BW.