This research presents the modeling and optimization of a magnetophoretic bio-separation chip for isolating cells, such as circulating tumor cells (CTCs), red, and white blood cells from the peripheral blood. The chip consists of a continuous flow through a microfluidic channel that contains locally engineered magnetic field gradients. The high gradient magnetic field produced by the magnets is spatially non-uniform and gives rise to an attractive force on magnetic particles moving through the channel. Simulations of the particle-fluid transport and the magnetic force were performed using the open-source software OpenFOAM to predict the trajectories and capture lengths of the particles within a fluidic channel. The computational model takes into account key forces, such as the magnetic and fluidic forces and their effect on design parameters for an effective separation. The results show that the microfluidic device has the capability of separating various cells with different sizes from their native environment. Additionally, to improve the performance of the separation device, a parametric study was performed to investigate the effects of the magnetic bead size, cell size, number of beads per cell, and flow rate on the cell separation performance. Computational results indicate that the trapping length decreases with increasing the number of beads per cell and the bead size. Also, the trapping length increased as the cell size was increased. Finally, an experimental study was performed to verify and validate the simulation results.
|Advisor:||Molki, Majid, Darabi, Jeff|
|School:||Southern Illinois University at Edwardsville|
|Department:||Mechanical and Industrial Engineering|
|School Location:||United States -- Illinois|
|Source:||MAI 56/01M(E), Masters Abstracts International|
|Subjects:||Biomedical engineering, Mechanical engineering, Biomechanics|
|Keywords:||Biomems, Biophysical techniques, Cell processes, Lab on a chip, Magnetic fields, Microfluidics|
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