Angiogenesis, the formation of new blood vessels, requires the reorganization of microtubules (MT), which are polar cytoskeletal structures, consisting of a free-plus end and a minus-end that is free or anchored. CAMSAP2 and CAMSAP3 have been shown to bind and stabilize MT minus-ends; yet, this activity's contribution to MT organization and directional migration is unknown. To investigate this contribution, we performed live-cell imaging of polarized HUVECs expressing CAMSAP2 or CAMSAP3. Our results show that CAMSAP2 and CAMSAP3 localized to the trailing edge of cells. Pharmacologic disassembly of MTs resulted in CAMSAP reorganization to the leading edge. MCAK expression is not sufficient for CAMSAP reorganization, but may recruit CAMSAP to the MT minus-end. MT growth dynamics analysis revealed that CAMSAP2 and CAMSAP3 promoted dynamic MT growth. These results suggest that CAMSAP2 and CAMSAP3 protect MTs against MCAK]mediated disassembly and also function to nucleate new, dynamic MTs at the leading edge.
|Advisor:||Myers, Kenneth A.|
|School:||University of the Sciences in Philadelphia|
|School Location:||United States -- Pennsylvania|
|Source:||MAI 56/01M(E), Masters Abstracts International|
|Subjects:||Biology, Cellular biology|
|Keywords:||CAMSAP2, CAMSAP3, Cellular migration, HUVEC, Microtubles|
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