The yeast Yarrowia lipolytica naturally produces ammonia and oils from protein substrates. The protein residue in the waste material from fermentations in a biorefinery can be utilized by this strain in order to produce additional bioproducts. In order to improve the amount of ammonia and oil produced, Y. lipolytica NRRL YB-567 was irradiated with UV-C and the resulting mutant strains were screened for ammonia production on plates containing bromothymol blue. Seven mutant strains were selected based on increased ammonia production (indicated by deep blue color) and colony density. The mutant strains were evaluated for ammonia production, protein utilization, growth rate, oil production, and 2-phenylethanol production. Oil composition was also evaluated for the mutant strain that produced the most oil relative to all other strains. Of the seven mutant strains, 5 produced more ammonia than the wild-type strain while only one strain produced more oil than the wild-type strain. The mutant strain has potential applications for use in biorefineries to produce valuable commercial products.
|Commitee:||Hughes, Stephen R., Morris, Sherri J., Stover, Naomi A.|
|School Location:||United States -- Illinois|
|Source:||MAI 56/01M(E), Masters Abstracts International|
|Subjects:||Biology, Genetics, Microbiology|
|Keywords:||High-throughput screening, Oleagenous yeast, Renewable products, UV mutagenesis, Yarrowia lipolytica|
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