The Pti1 kinase was identified from a reverse genetic screen as contributing to pattern-triggered immunity (PTI) against the bacterial speck pathogen Pseudomonas syringae pv. tomato (Pst). This was unexpected because Pti1 was originally identified as an interactor of the Pto kinase that confers effector-triggered immunity to Pst strains secreting either of the type III effectors AvrPto or AvrPtoB. A hairpin-Pti1 (hpPti1) construct was developed and used to generate stable transgenic tomato lines with reduced expression of Pti1. These hpPti1 plants were more susceptible to infection with Pst strains lacking both AvrPto and AvrPtoB and had reduced transcript accumulation of PTI-associated genes compared to wild-type plants. The hpPti1 plants produced less reactive oxygen species (ROS), but showed no difference in mitogen-activated protein kinase (MAPK) activation in response to two flagellin-derived peptides. Synthetic Pti1 genes designed to avoid silencing were transiently expressed in hpPti1 plants and restored the ability of the plants to produce wild-type levels of ROS. This work identifies a new component of PTI in tomato which, because it affects ROS production but not MAPK signaling, appears to act early in the immune response. We speculate that Pti1 was identified originally as a Pto interactor because it may interact with a Pto-like kinase that plays a role in PTI.
We identified several putative receptors in tomato that consist of extracellular malectin-like domains and intracellular kinase domains that show high similarity to Pto. Interestingly, the starting sequence of Pto and some of its family members is conserved in two of the identified malectin-like proteins, Mal1 and Mal2, at approximately the beginning of their kinase domains and is not found in any other predicted tomato protein. Silencing the orthologs of Mal1 and Mal2 in Nicotiana benthamiana resulted in compromised induction of immune responses and thus increased susceptibility to Pst infection. We found that AvrPtoB interacts with the kinase domains of both Mal1 and Mal2 and we speculate that these putative receptors are targeted by AvrPtoB to suppress plant immunity. Furthermore, we propose that Pto evolved from the kinase domain of either Mal1 or Mal2 (or a progenitor protein) to sense the presence of AvrPtoB inside the plant cell and induce robust defense responses.
We describe a rapid method to detect two major forms of fatty acylation, N-myristoylation and S-acylation, of candidate proteins using alkyne fatty acid analogs coupled with click chemistry. We applied our approach to confirm and decisively demonstrate that AvrPto, Pto, and the FLS2 receptor all undergo plant-mediated fatty acylation. In addition to providing a means to readily determine fatty acylation, particularly myristoylation, of candidate proteins, this method is amenable to a variety of expression systems. We demonstrate this using both Arabidopsis protoplasts and stable transgenic Arabidopsis plants and we leverage Agrobacterium-mediated transient expression in Nicotiana benthamiana leaves as a means for high-throughput evaluation of candidate proteins. The metabolic labeling approach leveraging alkyne fatty acid analogs and click chemistry described here has the potential to provide mechanistic details of the molecular tactics used at the host plasma membrane in the battle between plants and pathogens.
|Commitee:||Collmer, Alan, Mazourek, Michael|
|Department:||Plant Path and Plant-Microbe Bio|
|School Location:||United States -- New York|
|Source:||DAI-B 78/03(E), Dissertation Abstracts International|
|Subjects:||Genetics, Plant sciences, Plant Pathology, Biochemistry|
|Keywords:||Bacterial speck disease, Components, Innate immunity, Tomato|
Copyright in each Dissertation and Thesis is retained by the author. All Rights Reserved
The supplemental file or files you are about to download were provided to ProQuest by the author as part of a
dissertation or thesis. The supplemental files are provided "AS IS" without warranty. ProQuest is not responsible for the
content, format or impact on the supplemental file(s) on our system. in some cases, the file type may be unknown or
may be a .exe file. We recommend caution as you open such files.
Copyright of the original materials contained in the supplemental file is retained by the author and your access to the
supplemental files is subject to the ProQuest Terms and Conditions of use.
Depending on the size of the file(s) you are downloading, the system may take some time to download them. Please be