Weight loss is often prescribed to treat NAFLD; however, only one in six obese or overweight individuals who lose weight through diet are successful at maintaining weight loss resulting in weight regain (i.e., weight cycling). To determine the effect of physical activity on the prevention of hepatic steatosis and expression of lipogenic genes during weight cycling. To induce obesity, male C57BL/6 mice were fed a 60% fat diet for 10-weeks. Following weight gain, mice were randomly assigned to a 10% fat diet either with (WC+PA) or without (WC) physical activity to induce weight loss for 8 weeks. Physical activity consisted of unrestricted access to running wheels. Following weight loss, the Diet and WC+PA groups were switched back to a 60% fat diet for 10 weeks to cause weight regain. The WC+PA had continued access to physical activity during weight regain. Age-matched lean and obese control mice were fed either a 10% fat diet (LF) or 60% fat diet (HF) for the entire 28 weeks of the study. Significant differences (P<0.05) between groups were identified by one-way ANOVA. Following weight regain, body mass of the WC+PA was significantly lower than the HF (47.8 vs. 55.3 g) and WC (47.8 vs. 53.9 g). No significant difference in body mass was observed between WC and HF. The WC+PA had significantly lower plasma cholesterol levels compared to HF (230.5 vs. 254.5 mg/dL) and WC (230.5 vs. 271.9 mg/dL). In addition, the WC+PA group had significantly lower total hepatic lipid (23.2 vs. 26.5%) when compared with Diet, which was associated with 60%, 50%, and 40% lower expression of lipogenic genes Fasn, Srebp1c, and Chrebp, respectively. No difference was noted between WC and WC+PA for the expression of lipogenic genes Scd1 and Acc1. These data suggests that the continued physical activity during weight cycling resulted in lower weight regain and reduced the accumulation of hepatic lipid by decreased de novo lipogenesis. Overall, the reduced expression of lipogenic related genes might point to a potential protective mechanism that physical activity has on the development of NAFLD during weight cycling.
|Advisor:||Wooten, Joshua S.|
|Commitee:||Guilford, Brianne, Zuercher, Jennifer|
|School:||Southern Illinois University at Edwardsville|
|Department:||Kinesiology and Health Education|
|School Location:||United States -- Illinois|
|Source:||MAI 55/06M(E), Masters Abstracts International|
|Keywords:||Lipogenesis, Obesity, Weight cycling|
Copyright in each Dissertation and Thesis is retained by the author. All Rights Reserved
The supplemental file or files you are about to download were provided to ProQuest by the author as part of a
dissertation or thesis. The supplemental files are provided "AS IS" without warranty. ProQuest is not responsible for the
content, format or impact on the supplemental file(s) on our system. in some cases, the file type may be unknown or
may be a .exe file. We recommend caution as you open such files.
Copyright of the original materials contained in the supplemental file is retained by the author and your access to the
supplemental files is subject to the ProQuest Terms and Conditions of use.
Depending on the size of the file(s) you are downloading, the system may take some time to download them. Please be