Organizational trust is an important element of an organization’s success, as it is a central component of effective work relationships. This mixed-methods study examined the relationships between the perceived levels of trust that pastors have in a religious organization in the Midwestern region of the United States and for their current male regional bishops as the organizational leaders. Additionally, this research examined the difference between organizational trust and interpersonal trust based on gender. This study used the Organizational Trust Inventory (OTI) developed by Nyhan and Marlowe (1997) and qualitative interviews to measure trust. Results from the sample of 25 pastors revealed that there was a high level of organizational trust and interpersonal trust. When factoring in gender, the triangulation of data implied that the gender does not have a significant effect on the perceived level of organizational trust or interpersonal trust.