Systematic chemical analysis of cellular and tissue components is critical for understanding living organisms. The spatiotemporal distributions of transcripts, proteins, and metabolites characterize the functioning living systems during ontogenesis and in response to environmental perturbations. With high sensitivity, accuracy, and throughput, mass spectrometry (MS) has become an essential tool for studying the metabolism of plants, animals, and microbes. The introduction of electrospray as an ionization source has opened a door for the analysis and imaging of biological samples by MS under ambient conditions. This thesis describes my achievements in improving electrospray based ionization sources and extending the imaging capabilities of laser ablation electrospray ionization (LAESI) MS for the metabolomic study of various biological systems.
|Commitee:||Licht, Stuart, Massiah, Michael, Nita-Lazar, Aleksandra, Teng, H.Henry|
|School:||The George Washington University|
|School Location:||United States -- District of Columbia|
|Source:||DAI-B 77/11(E), Dissertation Abstracts International|
|Subjects:||Molecular biology, Cellular biology, Microbiology, Analytical chemistry|
|Keywords:||Ambient ionization, Electrospray, Imaging, Laser ablation, Laser ablation electrospray ionization, Mass spectrometry|
Copyright in each Dissertation and Thesis is retained by the author. All Rights Reserved
The supplemental file or files you are about to download were provided to ProQuest by the author as part of a
dissertation or thesis. The supplemental files are provided "AS IS" without warranty. ProQuest is not responsible for the
content, format or impact on the supplemental file(s) on our system. in some cases, the file type may be unknown or
may be a .exe file. We recommend caution as you open such files.
Copyright of the original materials contained in the supplemental file is retained by the author and your access to the
supplemental files is subject to the ProQuest Terms and Conditions of use.
Depending on the size of the file(s) you are downloading, the system may take some time to download them. Please be