Dissertation/Thesis Abstract

Image-guided precision manipulation of cells and nanoparticles in microfluidics
by Cummins, Zachary, Ph.D., University of Maryland, College Park, 2016, 128; 10129892
Abstract (Summary)

Manipulation of single cells and particles is important to biology and nanotechnology. Our electrokinetic (EK) tweezers manipulate objects in simple microfluidic devices using gentle fluid and electric forces under vision-based feedback control. In this dissertation, I detail a user-friendly implementation of EK tweezers that allows users to select, position, and assemble cells and nanoparticles. This EK system was used to measure attachment forces between living breast cancer cells, trap single quantum dots with 45 nm accuracy, build nanophotonic circuits, and scan optical properties of nanowires. With a novel multi-layer microfluidic device, EK was also used to guide single microspheres along complex 3D trajectories. The schemes, software, and methods developed here can be used in many settings to precisely manipulate most visible objects, assemble objects into useful structures, and improve the function of lab-on-a-chip microfluidic systems.

Indexing (document details)
Advisor: Shapiro, Benjamin
Commitee: Chen, Yu, Martin, Stuart, Waks, Edo, White, Ian
School: University of Maryland, College Park
Department: Bioengineering
School Location: United States -- Maryland
Source: DAI-B 77/10(E), Dissertation Abstracts International
Source Type: DISSERTATION
Subjects: Biomedical engineering, Nanotechnology
Keywords: CTCS, Circulating tumor cells, Lab-on-a-chip, Microfluidics, Nanowires, Quantum dots
Publication Number: 10129892
ISBN: 9781339877563
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