Behavioral sensitization is an increase in a behavioral response (e.g., locomotor activity or stereotypy) induced by previous drug exposure. The present study examined one-trial methamphetamine behavioral sensitization in male and female rats during early or late adolescence. During pretreatment, male and female rats received methamphetamine (0.0–6.0 mg/kg) in the home or in a novel chamber during early (PD 38) or late (PD 48) adolescence. After 24 hours, rats received a 1 mg/kg methamphetamine challenge test dose in the novel chamber to assess for sensitization. The results showed that rats in both age groups exhibited robust locomotor activity to the acute effects of methamphetamine. However, male and female rats at either age group did not exhibit one-trial methamphetamine behavioral sensitization. Overall, females exhibited greater locomotor activity than males, while males exhibited greater stereotypy. These findings do not provide evidence that the ontogeny of one-trial methamphetamine sensitization emerges during adolescence.