This study examined the collaborative aspects of instructional technology development and implementation on the P.R.I.D.E. Digital Curriculum (PDC). The multimedia PDC training product was developed through an inter-organizational collaboration amongst several agencies and organizations. An increase in globalization and decentralization of organizations is changing how instructional designers communicate with the design and development teams. Cultural differences between institutions is significant when designing and developing collaborative material. This study utilized activity theory and activity systems analysis to examine communication practices, cultural influences, and synergy of the partners with a developer’s perspective on instructional design and development. The findings revealed cultural differences did influence activities. The findings also revealed a historical influence on communication and decision-making practices. Additionally, the study uncovered some techniques used for improving synergistic properties while cautioning of antagonistic behaviors that threaten inter-organization collaboration.