Dissertation/Thesis Abstract

The Effect of Biglycan on TNF-alpha Signaling and Innervation in the Intervertebral Disc
by Buchanan, Samuel Andrew, M.S., Rush University, 2015, 96; 1581897
Abstract (Summary)

Biglycan is a small, leucine-rich repeat proteoglycan that has been shown to be elevated in the intervertebral discs (IVDs) of older adults (Cs-Szabo, 2002) and can interact with many different signaling molecules and structural proteins. TNF-alpha (TNF) is a general modulator of inflammation and may be a trigger in the chronic inflammatory response that leads to disc degeneration. Since biglycan has been shown to interact with TNFalpha (Tufvesson and Westergren-Thorsson, 2002), it may play a role in modulating its response in the IVD. Western (NFkB) and RT-PCR (MMP-1, -13, NGF and VEGF) assays were performed on both human and bovine cultured cells from IVD tissue. Treatment with biglycan alone was found to be almost indistinguishable from control groups; while biglycan in combination with TNF-alpha was able to significantly reduce transcription and expression response when compared with just TNF-alpha. Biglycan may help modulate the effects of TNF-alpha in the IVD.

Indexing (document details)
Advisor: Cs-Szabo, Gabriella
School: Rush University
School Location: United States -- Illinois
Source: MAI 55/03M(E), Masters Abstracts International
Subjects: Biochemistry
Keywords: Biglycan, Degeneration, Disc, Inflammation, TNF-alpha
Publication Number: 1581897
ISBN: 9781339396064
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