Dissertation/Thesis Abstract

The Identification of Alkaloid Pathway Genes from Non-Model Plant Species in the Amaryllidaceae
by Kilgore, Matthew Benjamin, Ph.D., Washington University in St. Louis, 2015, 186; 3745412
Abstract (Summary)

Secondary metabolites are often restricted in their distribution to different groups of organisms. For this reason, attempts to study these often useful and interesting products of metabolism require an ability to work in a diversity of non-model species. Methods for gene discovery with low investment and high efficiency are needed to effectively identify the biosynthetic genes in these diverse pathways. During this work, a workflow for efficiently identifying biosynthetic genes was developed and applied to Amaryllidaceae alkaloid biosynthesis. Genes discovered during this work include a norbelladine 4’- O-methyltransferase, a cytochrome P450 capable of phenol-phenol coupling 4’-O-methylnorbelladine to noroxomaritidine, and a short-chain dehydrogenase/reductase capable of forming norbelladine from tyramine and 3,4-dihydroxybenzaldehyde. These enzymatic discoveries support the future application of this workflow to other biosynthetic pathways and organisms.

Indexing (document details)
Advisor: Kutchan, Toni M.
Commitee: Haswell, Elizabeth, Henderson, Jeffrey, Jez, Joseph M., Kunkel, Barbara, Mockler, Todd
School: Washington University in St. Louis
Department: Biology and Biomedical Sciences (Plant and Microbial Biosciences)
School Location: United States -- Missouri
Source: DAI-B 77/05(E), Dissertation Abstracts International
Subjects: Plant sciences, Biochemistry
Keywords: Amaryllidaceae, Amaryllidaceae alkaloids, Cytochrome P450, Galanthus, Narcissus, Norbelladine
Publication Number: 3745412
ISBN: 978-1-339-38362-0
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