The emergence and spread of Verticillium wilt were observed in cotton plants at the R.R. Foil Plant Science Research Center at Mississippi State during the late summer in 2013 and 2014. Several fungi with different morphology and growth characteristics were isolated from diseased cotton plants. Genomic DNA was extracted from the isolated fungal species and used in molecular typing via PCR amplification and DNA sequencing analysis of the ribosomal internal transcribed spacer (ITS) region. A total of five fungal genera were identified, and Verticillium sp. was the most frequently isolated genus. The isolated Verticillium strains could be Verticillium dahliae, Verticillium longisporum or even Verticillium albo-atrum. A PCR-based genotyping method using VTA2 (Verticillium transcription activator) gene specific primers confirmed that the isolated Verticillium strain was Verticillium dahliae, and it caused Verticillium wilt in Mississippi cotton plants. Pathogenicity tests (Koch’s postulates) confirmed the earlier qualitative identifications of Verticillium dahliae in the greenhouse.
|Advisor:||Ma, Din-Pow, Lu, Shien|
|School:||Mississippi State University|
|Department:||Biochemistry and Molecular Biology|
|School Location:||United States -- Mississippi|
|Source:||MAI 55/02M(E), Masters Abstracts International|
|Subjects:||Molecular biology, Plant Pathology, Biochemistry|
|Keywords:||Cotton plant, Internal transcribed spacer region, VTA2, Vascular wilt, Verticillium dahliae, Verticillium wilt|
Copyright in each Dissertation and Thesis is retained by the author. All Rights Reserved
The supplemental file or files you are about to download were provided to ProQuest by the author as part of a
dissertation or thesis. The supplemental files are provided "AS IS" without warranty. ProQuest is not responsible for the
content, format or impact on the supplemental file(s) on our system. in some cases, the file type may be unknown or
may be a .exe file. We recommend caution as you open such files.
Copyright of the original materials contained in the supplemental file is retained by the author and your access to the
supplemental files is subject to the ProQuest Terms and Conditions of use.
Depending on the size of the file(s) you are downloading, the system may take some time to download them. Please be