The primary hypothesis of this project is that mobile genetic elements which facilitate horizontal gene transfer between closely related organisms should share essential elements required for replication and stable maintenance. To test this, several plasmids found in Rickettsia amblyommii AaR/SC were sequenced. The coding content of each was predicted and compared. Conserved regions were identified. Analysis of predicted open reading frames revealed that genes' functions that are well characterized on other plasmids also occur on the plasmids in Rickettsia. These genes ( dnaA, traA, and parA), which have functions related to stability and maintenance in other plasmids, were found to be present in the all the plasmids examined in this preliminary study and appeared to be fairly conserved and necessary for replication and stability.
|Advisor:||Williamson, Phillip C.|
|Commitee:||Budowle, Bruce, Chakraborty, Ranajit|
|School:||University of North Texas Health Science Center at Fort Worth|
|School Location:||United States -- Texas|
|Source:||MAI 55/02M(E), Masters Abstracts International|
|Keywords:||Plasmids, Recombinant dna, Rickettsia, Sequencing|
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