Dissertation/Thesis Abstract

Synthesis, characterization and biological evaluation of paclitaxel-hybrid peptides conjugates
by Hu, Chloe Y., M.S., California State University, Long Beach, 2015, 90; 1601316
Abstract (Summary)

A novel family of hybrid peptides consisting of cell penetrating peptides and collagen mimetic peptides was developed to be a promising drug carrier for paclitaxel. Conjugation of paclitaxel to lysine groups in hybrid peptides was successfully accomplished using HATU-mediated coupling with 17% yield and confirmed with HPLC, NMR, MALDI-TOF characterizations. Conformational changes of several hybrid peptides were examined using Circular Dichroism Spectroscopy. Their transition temperatures of unfolding (helix-to-coil transition) were determined ranging from 15.3 to 63.7 °C. We confirmed that the overall propensity of folding is highly dependent on the peptide sequence and number of amino acids in the sequence. The cytotoxicity of the paclitaxel conjugate was tested using cell proliferation assays. The IC50 of the paclitaxel conjugate (27.5 nM) was slightly higher than pure paclitaxel (15.8 nM), which indicates that the hybrid peptides are a promising class of drug carrier that enables the delivery of paclitaxel across the cellular membrane.

Indexing (document details)
Advisor: Slowinska, Katarzyna
Commitee: Schwans, Jason, Weers, Paul
School: California State University, Long Beach
Department: Chemistry and Biochemistry
School Location: United States -- California
Source: MAI 55/02M(E), Masters Abstracts International
Source Type: DISSERTATION
Subjects: Biochemistry
Keywords: Cell-penetrating peptides, Collagen peptides, Conjugation, Drug delivery, Paclitaxel, Triple helix
Publication Number: 1601316
ISBN: 9781339123875
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