The acculturation gap-distress hypothesis posits that discrepancies in cultural preferences and values among parents and their children in immigrant families lead to family conflict, further giving rise to child psychological distress. Studies examining this hypothesis have not independently examined parent-child gaps in specific acculturation domains among Mexican immigrant families. The main purpose of this study was to cross-sectionally examine whether parent-child acculturation gaps in the domains of language, cultural behaviors, cultural identification, and cultural values in the American culture and Mexican culture are associated with more family conflict and greater child psychological distress in Mexican immigrant families. The sample consisted of 84 Mexican/Mexican-American identified undergraduate students. Path analysis results indicated that larger parent-child acculturation gap in Mexican cultural values was associated with more family conflict; in turn, more family conflict was associated with greater child psychological distress. This study’s findings may help better understand the complex and diverse functions of acculturation gaps in Mexican immigrant families.