Many neurological diseases associated with cortical and hippocampal dysfunctions are sexually dimorphic in incidence and have been linked to defects in mRNA splicing. My thesis investigated the sex- and age-related changes in gene expression of six RNA processing genes within the cortex/hippocampus of male and female mice during early development. Gene expression was measured using RT-qPCR with mouse cortex/hippocampus samples collected on the day of birth and one week after birth. Immunoblotting was also used to measure the protein levels of one RNA processing gene to determine whether the expression paralleled mRNA expression. Dhx8 mRNA and Sf3a2 protein expression was found to be sexually dimorphic, while Thoc3, Tut1, and Sf3a2 showed mRNA expression changes with age. Sexually dimorphic and age-related expression of these genes suggests that RNA processing may play an important role in brain sexual differentiation and early neural development; however the underlying mechanisms remain to be elucidated.
|Commitee:||Sinchak, Kevin, Young, Kelly A.|
|School:||California State University, Long Beach|
|School Location:||United States -- California|
|Source:||MAI 54/06M(E), Masters Abstracts International|
|Subjects:||Molecular biology, Physiology, Developmental biology|
|Keywords:||Cortex/hippocampus, Early development, Mouse, Sexual differentiation|
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