Advancing age of the global population is resulting in an increase in the accumulation of age-related pathologies. Many potential molecular and cellular causes of aging are still being debated. Endogenous retroelements are a class of mobile DNA elements that have coexisted in the genomes of virtually every eukaryotic organism for much of evolution. Recent work indicates that retrotransposons are active in advanced age but their impact on aging has not been explored in detail. Evidence is accumulating to suggest that retrotransposon-mediated events are a potential source of genomic change that can influence aging. In this study I took advantage of a unique yeast model system to characterize aging in cells with or without Ty1 retroelements. I identify here a novel role of retroelements in extending yeast chronological lifespan in certain media conditions, rather than observing an anticipated pro-aging effect. This effect was correlated with changes in factors known to influence aging such as mitochondrial function, reactive oxygen species, and sensitivity to a chemical inhibitor of nutrient sensing pathways. These results demonstrate that the presence of these retroelements have unforeseen direct or indirect roles in influencing cellular processes relevant to lifespan. The ability of retrotransposons to regulate cellular functions has not been well investigated, in contrast to the ability of cellular pathways to regulate retrotransposons. The work here broadens our perspective on the role retrotransposons have in host cells to influence certain aspects of eukaryotic aging.
|Advisor:||Maxwell, Patrick H.|
|Commitee:||Collins, Cynthia, Conklin, Douglas S., Gilbert, Susan P.|
|School:||Rensselaer Polytechnic Institute|
|School Location:||United States -- New York|
|Source:||DAI-B 76/10(E), Dissertation Abstracts International|
|Keywords:||Aging, Hu, Retrotransposons, Ty1, Yeast|
Copyright in each Dissertation and Thesis is retained by the author. All Rights Reserved
The supplemental file or files you are about to download were provided to ProQuest by the author as part of a
dissertation or thesis. The supplemental files are provided "AS IS" without warranty. ProQuest is not responsible for the
content, format or impact on the supplemental file(s) on our system. in some cases, the file type may be unknown or
may be a .exe file. We recommend caution as you open such files.
Copyright of the original materials contained in the supplemental file is retained by the author and your access to the
supplemental files is subject to the ProQuest Terms and Conditions of use.
Depending on the size of the file(s) you are downloading, the system may take some time to download them. Please be