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Dissertation/Thesis Abstract

Design and Improvement of the Biosynthesis of 2,3–Butanediol from CO2 by Metabolic Engineering of Cyanobacterium Synechococcus elongatus PCC7942
by Oliver, John William Kidder, Ph.D., University of California, Davis, 2014, 145; 3685273
Abstract (Summary)

This dissertation describes metabolic engineering of cyanobacterium Synechococcus elongatus PCC7942 as a photosynthetic host for the conversion of CO2 into 2,3-butanediol. Current advances in pathway design, genetic tool development, and yield improvement are described (Chapter 1). A pathway for the synthesis of 2,3-butanediol is designed based on collective concepts of pathway strength, robustness, and irreversibility, and extensively tested through the generation of mutants (Chapter 2). This pathway is then optimized through modulation of translation by combinatorial mixing of ribosome binding sites (Chapter 3). Finally, photosynthetic productivity is investigated through expression of an exogenous pathway targeting every step between fixation and product (Chapter 4). All materials and methods are given separately for easy reference (Chapter 5).

Indexing (document details)
Advisor: Atsumi, Shota
Commitee: Chen, Xi, Toney, Michael
School: University of California, Davis
Department: Chemistry
School Location: United States -- California
Source: DAI-B 76/07(E), Dissertation Abstracts International
Subjects: Analytical chemistry, Biochemistry, Organic chemistry
Keywords: Cyanobacteria, Metabolic engineering, Renewable energy
Publication Number: 3685273
ISBN: 978-1-321-60958-5
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