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Dissertation/Thesis Abstract

An examination of the role of growth differentiation factor 15 (GDF15) in prevention of acute kidney injury
by Hernandez, Bryan Enrique, M.S., California State University, Los Angeles, 2014, 55; 1583068
Abstract (Summary)

Acute kidney injury (AKI) is characterized by a rapid decline of renal function that is associated with high mortality. Growth differentiation factor 15 (GDF15) has emerged as a therapeutic candidate of interest based on the rapid activation of GDF15 in a renal ischemia reperfusion injury (IRI) model of AKI. To explore the effects of GDF15 on renal repair, I utilized a Sleeping Beauty (SB) transposase plasmid to deliver a Gdf15 transposon by hydrodynamic tail vein injections into the mouse liver. One week later, mice were subjected to IRI to test the efficacy of pre-treatment with GDF15. Preliminary data show similar levels of kidney injury, assessed by serum creatinine levels, in experimental and control groups, though additional biological replicates are required for a definitive result. The general approach adopted here is a potential rapid and cost-effective strategy to generally examine the effects of secretory peptides in kidney injury and repair.

Indexing (document details)
Advisor: Nissen, Robert
Commitee: McMahon, Andrew, Nissen, Robert, Sharp, Sandra
School: California State University, Los Angeles
Department: Biological Sciences
School Location: United States -- California
Source: MAI 54/03M(E), Masters Abstracts International
Subjects: Molecular biology, Medicine
Keywords: GDF15, Hydrodynamic tail vein injection, Ischemia reperfusion injury, Ischemic acute kidney injury, Sleeping beauty transposase
Publication Number: 1583068
ISBN: 978-1-321-53458-0
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