Acute kidney injury (AKI) is characterized by a rapid decline of renal function that is associated with high mortality. Growth differentiation factor 15 (GDF15) has emerged as a therapeutic candidate of interest based on the rapid activation of GDF15 in a renal ischemia reperfusion injury (IRI) model of AKI. To explore the effects of GDF15 on renal repair, I utilized a Sleeping Beauty (SB) transposase plasmid to deliver a Gdf15 transposon by hydrodynamic tail vein injections into the mouse liver. One week later, mice were subjected to IRI to test the efficacy of pre-treatment with GDF15. Preliminary data show similar levels of kidney injury, assessed by serum creatinine levels, in experimental and control groups, though additional biological replicates are required for a definitive result. The general approach adopted here is a potential rapid and cost-effective strategy to generally examine the effects of secretory peptides in kidney injury and repair.
|Commitee:||McMahon, Andrew, Nissen, Robert, Sharp, Sandra|
|School:||California State University, Los Angeles|
|School Location:||United States -- California|
|Source:||MAI 54/03M(E), Masters Abstracts International|
|Subjects:||Molecular biology, Medicine|
|Keywords:||GDF15, Hydrodynamic tail vein injection, Ischemia reperfusion injury, Ischemic acute kidney injury, Sleeping beauty transposase|
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