Helicobacter is a genus of Gram-negative helical bacteria. Colonization location divides the genus into two groups: gastric and enterohepatic. Many species of Helicobacter have become associated with or cause gastric, intestinal, and hepatic disease. Helicobacter canadensisis an enterohepatic bacterium that has become associated with enteritis and bacteremia. The first part of this study investigated the intestinal inflammatory response to H. canadensis in mouse explants. Colon and cecum explants taken from C57BL/6J mice were incubated with 10
8 CFU/mL H. canadensis for 24 hours and cytokine ELISAs were conducted on supernatants. IL-6, IL-1alpha, and TNF-alpha were not induced in either colon or cecum explants. The second part of this study involved infecting thioglycollate-elicited macrophages with 10
8 CFU/mL H. canadensis for 6 or 24 hours followedby cytokine ELISAs of supernatants. At 6 hours, IL-6 secretion was increased in thioglycollate-elicited macrophages infected with H. canadensis while IL-1alpha and TNF-alpha secretion was undetectable. At 24 hours, IL-1alpha secretion was significantly greater in thioglycollate-elicited macrophages treated with H. canadensis while IL-6 and TNF-alpha secretion was undetectable. Colon and cecum explant data support previous findings in our lab that H. canadensis does not induce inflammation, while data collected from thioglycollate-elicited macrophage cultures indicate that H. canadensis stimulates inflammatory cytokines.
|Commitee:||Kitz, Dennis, Krajniak, Kevin|
|School:||Southern Illinois University at Edwardsville|
|School Location:||United States -- Illinois|
|Source:||MAI 54/03M(E), Masters Abstracts International|
|Keywords:||Explant, Helicobacter canadensis, Inflammatory cytokine, Macrophages|
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