Dissertation/Thesis Abstract

Development of a Direct Analysis Method for Copper in Serum by Tungsten Filament Electrothermal Atomic Absorption
by Shakya, Reema, M.S., Southern Illinois University at Edwardsville, 2014, 50; 1571929
Abstract (Summary)

The analysis of horse serum samples for copper is relevant to biomarkers for a variety of pathologies in humans. The inexpensive and compact nature of locally laboratory built tungsten filament electrothermal atomic absorption spectrometry instruments (WETAAS) makes them well suited to copper analysis in the clinical context; however, little research in this area exists. This project was undertaken to determine the experimental conditions necessary for determining copper in serum by WETAAS without resorting to sample digestion. Serum samples were prepared by 1:5 dilution in 0.2% nitric acid solution. The filament positioning with the light path plays an important role in the actual number of vaporized atoms of analyte being detected. In aqueous calibration standards, ammonium dihydrogen phosphate was found to be a critical matrix modifier, but in the presence of serum as an interference, the matrix modifier was not of much use. The results indicate that the serum matrix strongly suppresses the copper analytical signal. The merits of using integrated area for quantitation rather than peak height was found in the complex matrix system. Then the background concentration of copper in serum was determined by the standard additions method. Then the same samples were analyzed using a standard method for analysis of copper in serum through flame atomic absorption spectrometry, which is a matrix interference independent system.

Indexing (document details)
Advisor: Navarre, Edward C.
Commitee: Jones, Myron W., Voss, Eric J.
School: Southern Illinois University at Edwardsville
Department: Chemistry
School Location: United States -- Illinois
Source: MAI 54/02M(E), Masters Abstracts International
Source Type: DISSERTATION
Subjects: Neurosciences, Biochemistry
Keywords: Horse serum, Neurotransmitters
Publication Number: 1571929
ISBN: 9781321452266
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