Dissertation/Thesis Abstract

Search for Novel DNA Modifications in Saccharomyces cerevisiae mtDNA using Single Molecule Real Time Sequencing and Effects of Mitochondrial Metabolic Dynamics on Gene Expression
by Reinsborough, Calder, M.S., Icahn School of Medicine at Mount Sinai, 2014, 70; 1569125
Abstract (Summary)

In the past five years, Single Molecule Real Time (SMRT) sequencing technology has been found to be a reliable indicator of certain epigenetic modifications in bacterial genomes. The genome of the model organism Saccharomyces cerevisiae has long been thought to be free of DNA level modification, but literature surrounding this subject is conflicting. Additionally, the mitochondria of S. cerevisiae control the transition between three distinct chronological life phases – exponential, postdiauxic, and stationary - as defined by their main metabolic processes. This study attempted to identify base modifications to mtDNA using PacBio sequencing while additionally establishing gene expression changes as a result of altered mitochondrial metabolic capabilities. PacBio results showed intriguing results but statistical analysis proved experimentation with improved protocols were necessary. Multiple genes with unknown or uncharacterized function were also shown to have significant differential expression between metabolic life phases.

Indexing (document details)
Advisor: Chess, Andrew
Commitee: Fang, Gang, Mobbs, Charles
School: Icahn School of Medicine at Mount Sinai
Department: Neurosciences
School Location: United States -- New York
Source: MAI 54/01M(E), Masters Abstracts International
Subjects: Cellular biology, Bioinformatics, Developmental biology
Keywords: DNA modification, Epigenetics, Lifespan, Pacbio, Rnaseq, Yeast
Publication Number: 1569125
ISBN: 9781321343373
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