Polychlorinated biphenyls (PCBs) negatively affect reproduction across taxa. However, how PCBs influence elasmobranchs is not well known. I hypothesized that acute PCB exposure would negatively impact reproductive parameters of newly-mature round stingrays (Urobatis halleri). To determine the acute effects of PCBs on steroidogenesis, male stingrays were injected with either Aroclor-1254 or vehicle (sesame-oil) and held for 24 hrs. Testicular mRNA expression for steroidogenic acute regulatory protein and 3β-hydroxysteroid dehydrogenase decreased with 24 hrs exposure to Aroclor-1254 (p<0.05). To test sperm quality, seminal fluid was exposed to Aroclor-1254 (0.00, 0.001, 0.010, 0.100, 1.000, 10.00 mg/L) for < 1 or 24 hrs. Aroclor-1254 (10.00 mg/L) decreased spermatozoa motility and viability at both time-points compared to controls (p<0.001). At < 1 hrs, spermatozoa motility decreased from the control at 1.000 mg/L (p<0.05) and viability decreased at 0.010 mg/L (p<0.05). These results indicate that Aroclor-1254 may have targeted effects on U. halleri, inhibiting spermatozoa function and steroidogenic enzyme activity at an acute dose.
|Advisor:||Young, Kelly A., Lowe, Christopher G.|
|Commitee:||Kelley, Kevin M.|
|School:||California State University, Long Beach|
|School Location:||United States -- California|
|Source:||MAI 53/06M(E), Masters Abstracts International|
|Subjects:||Genetics, Zoology, Environmental Studies|
|Keywords:||Environmental toxicology, Polychlorinated biphenyls, Shark reproduction, Stingray, Urobatis halleri|
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