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Magnetic resonance imaging (MRI) is a non-invasive method for imaging and diagnosing tissue damage, organ function and the vascular system. Magnevist™ a complex of diethylenetriaminepentaacetic acid (DTPA) and Gd3+ is a clinically approved contrast agent for MRI. A derivative of DTPA was formed by the addition of two cysteine groups (DTPA-L-Cys) through amide linkage. The Gd complex of this ligand bonds with the silver surfaces through the cysteine thiols. GdDTPA-L-Cys was bound to ∼10nm diameter Ag nanoparticles for use as a multifunctional MRI contrast agent. The ligand and complex were characterized by 1H and 13C NMR, ESI-MS and IR spectroscopy. The silver construct was characterized by TEM, TGA and UV-Vis absorption spectra. The per metal complex r1 relaxivity of GdDTPA-L-Cys{Ag} greater than that of Magnavist™ with the same molarity for both compounds.
The synthesis of a DTPA derivative is described that allows it to bind to silver or gold nanoparticles through a single thiol linkage (DTPASH). The resulting Gd complex, GdDTPASH, was bound to Ag nanoparticles to create a single monolayer on the surface. The construct was further stabilized in buffered solution with the addition of a thiolated PEG chain. The highly stabilized nanoparticle construct delivers a high payload of Gd compelex and is an effective T1 brightening agent. The production of this type of construct opens the way for engineered multimodal MRI contrast agents.
Advisor: | Walters, Marc A. |
Commitee: | Jerschow, Alexej, Mahal, Lara, Weck, Marcus, Zaim-Wadghiri, Youssef |
School: | New York University |
Department: | Chemistry |
School Location: | United States -- New York |
Source: | DAI-B 76/02(E), Dissertation Abstracts International |
Source Type: | DISSERTATION |
Subjects: | Organic chemistry, Nanoscience |
Keywords: | Contrast agent, GDDTPA, Magnetic resonance imaging, Multivalent, Nanoparticle, Silver |
Publication Number: | 3638474 |
ISBN: | 978-1-321-22092-6 |