Dissertation/Thesis Abstract

Delmopinol-induced matrix removal facilitates photodynamic therapy and chlorhexidine methods for disinfecting mixed oral biofilms
by Rogers, Stephen Christopher, M.S., State University of New York at Buffalo, 2014, 127; 1561655
Abstract (Summary)

It is often observed that the slimy matrixes of various bacterial-formed biofilms can limit their disinfection. This investigation demonstrated that disinfection effectiveness by either photodynamic therapy (PDT) or chlorhexidine irrigation is significantly improved by collapse of that matrix using the non-bactericidal reagent delmopinol as part of the treatment sequence. Cyclic shear-producing conditions were used to grow 4-day, whole salivary and growth media biofilms on glow-discharge-treated polystyrene (N=46) and mini-germanium internal reflection prisms to serve in a periodontal crypt model of disinfection by either methylene-blue-mediated PDT or by chlorhexidine irrigation. Assays for bacterial viability, with and without treatments, were performed by alamarBlue® fluorescent methods, statistically applied (ANOVA, Tukey's HSD). Multiple Attenuated Internal Reflection Infrared (MAIR-IR) assays confirmed selective removal of the predominantly polysaccharide matrix materials by the delmopinol treatment, but not by equivalent water or chlorhexidine methods. Confocal-IR microscopy showed that the delmopinol reagent, alone, caused about one-third of each wet biofilm to be removed, while bacterial re-growth was confirmed by alamarBlue® assay. Chlorhexidine and PDT suppression of bacterial activity without regrowth was significantly improved with the added delmopinol treatment, and is likely to provide similarly beneficial results in the effective disinfection of diverse biofilms in many settings.

Indexing (document details)
Advisor: Baier, Robert E.
Commitee: Andreana, Sebastiano, Mang, Thomas S.
School: State University of New York at Buffalo
Department: Biomaterials
School Location: United States -- New York
Source: MAI 53/04M(E), Masters Abstracts International
Subjects: Biology, Dentistry, Materials science
Keywords: Biofilm, Chlorhexidine, Delmopinol, Exopolysaccharide, Oral bacteria, Photodynamic therapy
Publication Number: 1561655
ISBN: 978-1-321-07218-1
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