This thesis presents the modeling, design, fabrication, and testing of a magnetophoretic bioseparation chip for isolation of biomaterials such as cells, antigens or DNA from their native environment. This microfluidic-based bioseparation device has several unique features, including locally engineered magnetic field gradients and a continuous flow with a buffer switching scheme to improve the performance of the separation process. The overall dimensions of the device are 25 mm by 75 mm by 1 mm. The cell purity was found to increase with increasing the sample flow rate. However, the cell recovery decreases with an increase in the flow rate. A compressive parametric study is performed to investigate the effects of channel height, substrate thickness, magnetic bead size, cell size, flow rate, and the number of beads per cell on the cell separation performance.
|Commitee:||Celik, Serdar, Molki, Majid|
|School:||Southern Illinois University at Edwardsville|
|Department:||Mechanical and Industrial Engineering|
|School Location:||United States -- Illinois|
|Source:||MAI 53/03M(E), Masters Abstracts International|
|Subjects:||Engineering, Mechanical engineering, Biomechanics|
|Keywords:||Bioseparation, Chip, Magnetrophoretism, Microfluid, Superparamagnetism|
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