Dissertation/Thesis Abstract

Phylogenetic analysis, modeling and experimental studies of the Saccharomyces cerevisiae palmitoylated protein kinase gene, ENV7
by Cocca, Stephanie M., M.S., California State University, Long Beach, 2014, 158; 1527907
Abstract (Summary)

Env7 is a vacuole membrane-localized protein kinase that is orthologous to the human serine/threonine protein kinase, STK16. It is evolutionarily well-conserved throughout Eukarya, and it has one ortholog in Bacteria. Phylogenetic analyses of sequences homologous to Env7 revealed clades that are inconsistent with established eukaryotic phylogeny, suggesting that both horizontal and vertical gene transmission are responsible for their conservation. Conserved amino acid residues and motifs that are potentially important to Env7's catalytic activity, localization, and interactions with other proteins were also identified and assessed. Additionally, one such conserved motif—the glycine-rich loop—was mutated in an effort to affect ATP binding in Env7. The phenotype resulting from this mutation was a slightly increased number of mutant cells exhibiting multi-lobed vacuoles under normal conditions.

Indexing (document details)
Advisor: Gharakhanian, Editte
Commitee: Brusslan, Judy, Malcomber, Simon
School: California State University, Long Beach
Department: Biological Sciences
School Location: United States -- California
Source: MAI 53/02M(E), Masters Abstracts International
Subjects: Molecular biology, Microbiology, Bioinformatics
Keywords: Kinase, Phylogenetics, Saccharomyces cerevisiae, Yeast
Publication Number: 1527907
ISBN: 978-1-303-98431-0
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