Leaf senescence, the orderly dismantling of plant tissue that promotes nutrient recycling, is accompanied by drastic changes in gene expression which may be regulated by post-translational modifications of histones such as acetylation. In this study, chromatin immunoprecipitation (ChIP) with an anti-acetylated histone antibody and ChIP sequencing (ChIP-seq) were used to perform a genome-wide analysis of histone 3 lysine 9 (H3K9) acetylation during leaf senescence. Those data were compared to genome-wide analysis of mRNA expression (RNA-seq). The results showed that changes in H3K9 acetylation were occurring, but these events were infrequent during leaf senescence. Furthermore, on a global scale, a strong correlation between H3K9 acetylation and gene expression during senescence was not observed. However, there was a small group of genes that associated with significantly changed acetylated H3K9 proteins and showed differential expression. Many of those genes were involved in biological functions that are up- or down-regulated during leaf senescence.
|Commitee:||Eldon, Elizabeth, Tsai, Houng-Wei|
|School:||California State University, Long Beach|
|School Location:||United States -- California|
|Source:||MAI 53/01M(E), Masters Abstracts International|
|Subjects:||Plant biology, Genetics, Biochemistry|
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