Ring canals are made from arrested cleavage furrows, and provide direct cytoplasmic connections among sibling cells. They are well documented for their participation in Drosophila oogenesis, but little is known about their role in several somatic tissues in which they are also found. Using a variety of genetic tools in live and fixed tissue, I demonstrate that the ∼250 nm diameter somatic ring canals permit rapid intercellular exchange through somatic ring canals by diffusion. Additionally, intercellular diffusion of protein was observed between cells with highly disparate levels of mRNA transcript, suggesting a possible role for ring canals in smoothing gene expression within a tissue. I also used a novel combination of markers to evaluate the extent of protein movement within mitotic clones and across clone boundaries in ovarian follicle cells and imaginal discs, providing evidence of robust movement of GFP between the two sides of mitotic clones and frequently into non-recombined cells. These data suggest that, depending on the experimental setup and proteins of interest, inter-clonal diffusion of protein may alter the interpretation of clonal data in follicle cells. Our work illustrates the lack of cytoplasmic autonomy in these tissues and suggests a role for somatic ring canals in promoting homogeneous protein expression within the tissue.
|School Location:||United States -- Connecticut|
|Source:||DAI-B 75/09(E), Dissertation Abstracts International|
|Subjects:||Genetics, Cellular biology, Developmental biology|
|Keywords:||Cell division, Cytokinesis, Drosophila, Follicle cells, Intercellular bridge, Ring canal|
Copyright in each Dissertation and Thesis is retained by the author. All Rights Reserved
The supplemental file or files you are about to download were provided to ProQuest by the author as part of a
dissertation or thesis. The supplemental files are provided "AS IS" without warranty. ProQuest is not responsible for the
content, format or impact on the supplemental file(s) on our system. in some cases, the file type may be unknown or
may be a .exe file. We recommend caution as you open such files.
Copyright of the original materials contained in the supplemental file is retained by the author and your access to the
supplemental files is subject to the ProQuest Terms and Conditions of use.
Depending on the size of the file(s) you are downloading, the system may take some time to download them. Please be