Dissertation/Thesis Abstract

Chromatin modifiers in Xenopus laevis: Protein arginine methyltransferase 5 function and Williams syndrome transcription factor complexes in development
by Stopa, Nicole, M.S., University of Alaska Anchorage, 2014, 126; 1556018
Abstract (Summary)

Cellular DNA is condensed and stored with the help of proteins, especially histones. This mix of DNA and proteins is termed chromatin. Manipulations of chromatin include post-translational modifications (PTMs) of histone proteins, such as the addition of methyl or acetyl groups, and the movement of histones by chromatin remodeling complexes to control how tightly or which regions of DNA are condensed or exposed. These actions can impact gene expression and thus influence the differentiation of tissues during development. I investigated if the enzyme protein arginine methyltransferase 5 (PRMT5), which mono- or symmetrically dimethylates arginine, is acting on histones during early development in Xenopus laevis. I also investigated if Williams syndrome transcription factor (WSTF) occurs within chromatin remodeling complexes during early development in X. laevis. WSTF interacts with the protein ISWI in early embryos, indicating it is part of a chromatin remodeling complex during this period of development.

Indexing (document details)
Advisor: Krebs, Jocelyn E.
Commitee: Harrison, Benjamin R., Hinterberger, Timothy J.
School: University of Alaska Anchorage
Department: Biological Sciences
School Location: United States -- Alaska
Source: MAI 52/06M(E), Masters Abstracts International
Subjects: Biology, Molecular biology, Developmental biology
Keywords: Chromatin, Chromatin remodelers, Prmt5, Protein arginine methyltransferase 5, Williams syndrome, Wstf
Publication Number: 1556018
ISBN: 9781303896293
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