The purpose of this study was to investigate the effects of Green Coffee Bean Extracts (GCBE) on fat metabolism (adiopogenesis and lipolysis) on 3T3-L1 preadipocytes in vitro. The cells were cultured and treated with GCBE at two different points of the adipocyte maturation life cycle—on day 0 (experiment design A) and on day 7 (experiment design B). In both experiment designs, samples 500 μg/ml GCBE, 50 μg/ml Caffeine, and 250 μg/ml Chlorogenic acid were compared to the control. The adipogenic effects were assessed by Western Blot using PPARγ and CEBPα as the adipogenic gene expression markers. Lipolytic effects were assessed by Lipolysis assay and were visualized by Oil Red 0 staining. Western Blot reading indicated GCBE had no significant adipogenic effect. Lipolysis Assay revealed that GCBE-treated cells were significantly reduced at p < 0.001. GCBE significantly suppressed fat-breakdown which was opposite of what was expected. These results convey a possibility that GCBE might not work as a potentially effective weight-reducing nutraceutical, and alerts consumers of a probable counteracting weight-gaining effect from the GCBE supplements available on market. Further studies are still needed to elucidate its mechanisms and the mode of action in vivo.
|School:||California State University, Long Beach|
|School Location:||United States -- California|
|Source:||MAI 52/06M(E), Masters Abstracts International|
|Subjects:||Molecular biology, Nutrition, Pharmacy sciences|
|Keywords:||3T3-L1, Caffeine, Chlorogenic acid, Fat metabolism, Green coffee beans, Weight loss|
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