Our studies examined the antioxidant properties of two classes of compounds, N-methyl-D-Aspartate receptor (NMDAR) antagonists and radiosensitizers. Antioxidant properties can augment or attenuate desired functions of these compounds, which are their abilities to treat neurodegenerative diseases and cancers, respectively. We evaluated the capability of each compound to scavenge reactive oxygen species (ROS) using chemical methods, i.e. nitric oxide (NO) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical activity. We also examined the compounds using biological methods, i.e. cell culture studies utilizing human breast adenocarcinoma cell line, MCF-7 assays. The results showed insignificant decrease of free radical production for radiosensitizers and some of NMDAR antagonists. An NMDAR atagonist, (compound 9) showed a high percentage of antioxidant activity at 1 mM and cell viability at 10 µM. Future studies can estimate ROS levels in cell culture, especially in neuronal cell lines. We can also screen more compounds for desired activities. iv
|Commitee:||Bruist, Michael F., Li, Zhiyu, McEwen, Charles N|
|School:||University of the Sciences in Philadelphia|
|School Location:||United States -- Pennsylvania|
|Source:||MAI 52/05M(E), Masters Abstracts International|
|Keywords:||Antagonists, Antioxideant properties, Breast cancer cell line, Free radicals, N-methyl-D-Aspartate receptor, Nitric oxide, Radiosensitizers, Reactive oxygen species|
Copyright in each Dissertation and Thesis is retained by the author. All Rights Reserved
The supplemental file or files you are about to download were provided to ProQuest by the author as part of a
dissertation or thesis. The supplemental files are provided "AS IS" without warranty. ProQuest is not responsible for the
content, format or impact on the supplemental file(s) on our system. in some cases, the file type may be unknown or
may be a .exe file. We recommend caution as you open such files.
Copyright of the original materials contained in the supplemental file is retained by the author and your access to the
supplemental files is subject to the ProQuest Terms and Conditions of use.
Depending on the size of the file(s) you are downloading, the system may take some time to download them. Please be