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Dissertation/Thesis Abstract

Binding analysis of apolipophorin III to lipopolysaccharides
by Pratt, Cynthia C., M.S., California State University, Long Beach, 2014, 69; 1527576
Abstract (Summary)

Apolipophorin III is a small apolipoprotein responsible for lipid transport in adult insects. Biological studies are beginning to show that apolipophorin III is also capable of detoxifying lipopolysaccharides. Lipopolysaccharides are found in the cell wall of gramnegative bacteria and are responsible for toxicity during sepsis. This research aims to (1) establish a binding interaction of lipopolysaccharides and apolipophorin III and (2) gain insight into the biochemical basis for that interaction. PAGE analysis and gel filtration chromatography was used to show that a complex was formed between apolipophorin III and lipopolysaccharides. A tyrosine fluorescence assay was used to obtain more details of the binding interaction between apolipophorin III and lipopolysaccharide. This indicated that binding between apolipophorin III and lipopolysaccharide occurs not only between smooth lipopolysaccharides but also between detoxified lipopolysaccharides. This showed that apoLp-111 binds to the carbohydrate portion oflipopolysaccharides as well as the lipid portion.

Indexing (document details)
Advisor: Weers, Paul
School: California State University, Long Beach
School Location: United States -- California
Source: MAI 52/05M(E), Masters Abstracts International
Subjects: Biochemistry
Publication Number: 1527576
ISBN: 978-1-303-79615-9
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