Reports in the literature indicate that high incidences of cancer in certain species of marine mammals may be linked to exposure to elevated levels of anthropogenic pollutants. This study aimed to determine whether stranded California sea lions with cancer exhibited higher levels of xenobiotic induced biomarkers of stress compared to animals without cancer. Pollutant concentrations in tissues were measured using GC-MS. CYP1A gene induction, protein content and activity were measured using RT-PCR, Western immunoblot and EROD assays, respectively. BPDE adducts were measured using ELISAs. BPDE-protein adducts were the only measured factor associated with cancer. Pollutants were positively correlated to gene induction. TEQ, gene induction, protein content and EROD activity were negatively correlated to BPDE-protein adducts in certain tissues. Based on these results involving a limited number of samples, we were unable to clearly associate cancer with xenobiotic exposure.
|Advisor:||Mason, Andrew Z.|
|Commitee:||Goodmanlowe, Gwen, Gossett, Rich, Kelley, Kevin|
|School:||California State University, Long Beach|
|School Location:||United States -- California|
|Source:||MAI 52/05M(E), Masters Abstracts International|
|Subjects:||Genetics, Zoology, Animal sciences, Oncology|
|Keywords:||California sea lions, Cancer, Pollutants, Zalophus californianus|
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