myo-Inositoloxygenase (MIOX) catalyzes the first step in myo-inositol catabolism. MIOX has not been annotated in Drosophila melanogaster, but the protein encoded by the CG6910 gene is similar to the mouse MIOX protein. CG6910 "knocked-down" expression was explored using RNAi. "Knock-down" flies did not survive on inositol defined media, indicating that CG6910 encodes MIOX. Survival of these flies on sucrose defined media suggest that MIOX is not essential for development. Biochemical assays demonstrated that D. melanogaster has MIOX activity. Computational analyses revealed potential miRNA sites, and that a number of essential components are conserved. MIOX genes found in other drosopholids are highly similar to D. melanogaster MIOX, and analyses of the syntenic regions concur with established evolution. Western blot analyses showed differential expression amongst D. melanogaster from different geographic locations and between species. These studies may contribute to understanding the role of inositol catabolism in fruit fly development and diabetes.
|Advisor:||Klig, Lisa S.|
|Commitee:||Carter, Ashley J. R., Eldon, Elizabeth D.|
|School:||California State University, Long Beach|
|School Location:||United States -- California|
|Source:||MAI 52/05M(E), Masters Abstracts International|
|Keywords:||Catabolism, Drosophila melanogaster, Inositol|
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