myo-Inositol plays an important role in osmoregulation, spinal cord development and diseases such as Alzheimer’s, autism, Down syndrome and bipolar disorder. myo- Inositol-3-phosphate synthase (MIPS) converts glucose-6-phosphate to myo-inositol-3- phosphate. In Drosophila melanogaster MIPS has a computationally determined molecular weight (MW) of 62 kDa and is 59% identical (72% similar) to the human MIPS protein. This study demonstrated the presence of multiple isoforms of the MIPS protein in D. melanogaster. Specifically, western blot analyses reveal MIPS isoforms of approximately 64 kDa, 63 kDa, 62 kDa and 40 kDa. These multiple MIPS isoforms are expressed in a gender specific manner. This study also showed that a partial deletion of the D. melanogaster MIPS gene results in a lethal phenotype, suggesting that inositol synthesis is essential for proper growth and development of D. melanogaster. These conclusions may contribute to understanding the role of inositol synthesis in development and neurological disorders.
|Advisor:||Klig, Lisa S.|
|School:||California State University, Long Beach|
|School Location:||United States -- California|
|Source:||MAI 52/05M(E), Masters Abstracts International|
|Subjects:||Molecular biology, Genetics, Developmental biology|
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