Proteins are molecular machines of life in the truest sense. Being the expressors of genotype, proteins have been a focus in structural biology. Since the first characterization and structure determination of protein molecule more than half a century ago1, our understanding of protein structure is improving only incrementally. While computational analysis and experimental techniques have helped scientist view the structural features of proteins, our concepts about protein folding remain at the level of simple hydrophobic interactions packing side-chain at the core of the protein. Furthermore, because the rate of genome sequencing is far more rapid than protein structure characterization, much more needs to be achieved in the field of structural biology. As a step in this direction, my dissertation research uses computational analysis and experimental techniques to elucidate the fine structural features of the tertiary packing in proteins. With these set of studies, the knowledge of the field of structural biology extends to the fine details of higher order protein structure.
|Advisor:||Tsai, Jerry W.|
|Commitee:||LiWang, Andy, LiWang, Patricia, McCallum, C. Michael, Tsai, Jerry W., Xue, Liang|
|School:||University of the Pacific|
|Department:||Pharmaceutical and Chemical Sciences|
|School Location:||United States -- California|
|Source:||DAI-B 75/05(E), Dissertation Abstracts International|
|Subjects:||Biochemistry, Bioinformatics, Biophysics|
|Keywords:||Casp, Cd, De novo sequence design, Expression, Helix-helix, Helix-sheet, Knob-socket model, Nmr, Protein design, Protein structure, Purification, Structure prediction, Tertiary packing|
Copyright in each Dissertation and Thesis is retained by the author. All Rights Reserved
The supplemental file or files you are about to download were provided to ProQuest by the author as part of a
dissertation or thesis. The supplemental files are provided "AS IS" without warranty. ProQuest is not responsible for the
content, format or impact on the supplemental file(s) on our system. in some cases, the file type may be unknown or
may be a .exe file. We recommend caution as you open such files.
Copyright of the original materials contained in the supplemental file is retained by the author and your access to the
supplemental files is subject to the ProQuest Terms and Conditions of use.
Depending on the size of the file(s) you are downloading, the system may take some time to download them. Please be