Psoriasis is a chronic inflammatory disease characterized by hyperproliferation and abnormal differentiation of keratinocytes. Curcumin, a constituent of the plant turmeric, is known to have anti-inflammatory effects in psoriatic HaCaT cell models stimulated by lipopolysaccharide (LPS). However, the anti-inflammatory effects of curcumin have yet to be tested against the anti-inflammatory effects of the whole root turmeric on LPS stimulated HaCaT cell model. In the present study, we tested the inflammatory response of curcumin versus turmeric in LPS and interleukin-6 (IL-6) stimulated psoriatic cells. IL-6 is a known stimulator of common psoriasis inflammatory pathways such as NF-kB and Jak/STAT3. Results showed TNF-α production was very low in both LPS and IL-6 stimulated treatments, suggesting that TNF-α may not be the best inflammatory stimulatory in a HaCaT psoriatic skin model. Single samples were independently tested, media only, LPS only, turmeric only, curcumin only, LPS/turmeric 4.75μM and LPS/curcumin 4.75μM, on known psoriatic inflammatory cytokines to determine cytokine levels. Outside testing results suggest that turmeric had anti-inflammatory effects by acting as a pro-inflammatory for IL-12p40 inhibiting IFN-γ from activating inflammatory pathways. Curcumin had pro-inflammatory effects on IFN-γ and IL-6, and anti-inflammatory effects on IL-12p40 and IL-1β. Both treatments had pro and anti-inflammatory effects on known psoriatic cytokines. These results imply that psoriatic disease has a balance of pro- and anti-inflammatory responses both of which curcumin and turmeric are capable of eliciting.
|Advisor:||Lund, Kaleb C.|
|Commitee:||Kazaks, Alexandra, Messner, Don|
|Department:||Department of Nutrition|
|School Location:||United States -- Washington|
|Source:||MAI 52/04M(E), Masters Abstracts International|
|Keywords:||Hacat, Il-6, Inflammation, Lps, Psoriasis, Tnf-alpha|
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