Reclaiming wastewater is increasing in the US to combat dwindling freshwater supplies. This water potentially contains pathogenic bacteria; therefore, we evaluated the occurrence, concentration, and antimicrobial susceptibilities of Enterococcus spp.--an important opportunistic pathogen that remains a leading cause of nosocomial infections--in reclaimed water used for spray irrigation (SI). A total of 48 wastewater effluent and SI samples were collected in 2009 and 2010 from the Mid-Atlantic and Midwest regions of the US. Enterococci were isolated using membrane filtration, confirmed using biochemical tests and PCR, and tested for antimicrobial susceptibility using the Sensititre® dilution system. We detected total enterococci and vancomycin-resistant enterococci (VRE) in 68% (27/40) and 8% (3/40), respectively, of all SI samples. VRE and vancomycin-intermediate enterococci (VIE) represented 2% (1/41) and 10% (4/41), respectively, of the total enterococci recovered from all SI sites. Our findings show that SI workers may be exposed to enterococci during spray irrigation activities.
|Advisor:||Sapkota, Amy R., Turner, Paul|
|School:||University of Maryland, College Park|
|Department:||Maryland Institute for Applied Environmental Health|
|School Location:||United States -- Maryland|
|Source:||MAI 52/03M(E), Masters Abstracts International|
|Subjects:||Microbiology, Environmental Health, Public health, Environmental science|
|Keywords:||Antibiotic resistance, Enterococcus, Multidrug resistance, Reclaimed wastewater, Spray irrigation|
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