Dissertation/Thesis Abstract

Diversity and selection in the major histocompatibility complex: DQA and immune function in IRL and Atlantic bottlenose dolphines (Tursiops truncatus)
by Ferrer, Tatiana, M.S., Florida Atlantic University, 2013, 86; 1524502
Abstract (Summary)

The Major Histocompatibility Complex (MHC) encodes proteins critical to the vertebrate immune response; therefore MHC diversity is an indicator of population health. I have (1) Isolated exon 2 of the class II gene DQA in Tursiops truncatus in the North Indian River Lagoon (IRL) (n=17), South IRL (n=29) and adjacent Atlantic waters (n=20), (2) assessed genetic variability between groups, (3) developed a method to genotype individuals, (4) typed 11 unique alleles in 66 individuals, (5) detected geographic patterns of diversity between estuarine and coastal individuals (FST=0.1255, p≤0.05), (6) found evidence of positive selection centered in the binding pockets P1, P6 and P9 of the peptide binding region (ω=2.08), (7) found that patterns of polymorphism did not closely match patterns of diversity in neutral markers, (8) performed a pilot study with Orcinus orca . The initial findings highlight the need for further comparative work and suggest that silent mutations are not neutral.

Indexing (document details)
Advisor: O'Corry-Crowe, Greg
School: Florida Atlantic University
School Location: United States -- Florida
Source: MAI 52/03M(E), Masters Abstracts International
Subjects: Molecular biology, Genetics, Conservation
Publication Number: 1524502
ISBN: 978-1-303-55500-8
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