In the present study, a form of endocrine disruption that impacts production of the important stress and metabolic hormone, cortisol, was confirmed in southern California English sole (Parophrys vetulus) and further characterized using cellular and molecular approaches. One of the key initial findings of the study was that the interrenal itself, when tested directly for its in vitro response to pituitary ACTH, was non-responsive in fish exhibiting the endocrine-disrupted phenotype. This pointed directly to a dysfunctional interrenal gland in the impacted fish. With this knowledge in hand, a proteomics-based approach was employed to screen for changes in tissue protein expression. This experimental effort resulted in the molecular identification of 107 proteins, 83 of which were from unique genes, and it elucidated a variety of changes in protein expression related to interrenal function and to exposure of the fish to specific kinds of anthropogenic environmental chemicals.
|Advisor:||Kelley, Kevin M.|
|Commitee:||Allen, Bengt J., Armstrong, Jeffrey L., Livingston, Brian|
|School:||California State University, Long Beach|
|School Location:||United States -- California|
|Source:||MAI 52/03M(E), Masters Abstracts International|
|Subjects:||Endocrinology, Environmental Health, Physiology, Aquatic sciences|
|Keywords:||California, Parophrys vetulus|
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