Dissertation/Thesis Abstract

Estradiol dose dependently regulates membrane estrogen receptor-α and metabotropic glutamate receptor-1a complexes in the arcuate nucleus of the hypothalamus
by Mahavongtrakul, Matthew, M.S., California State University, Long Beach, 2013, 57; 1524139
Abstract (Summary)

Sexual receptivity in the female rat is dependent on dose and duration of estradiol exposure. A 2µg dose of estradiol benzoate (EB) primes reproductive behavior circuits but without subsequent progesterone does not facilitate lordosis. However, 50µg EB facilitates lordosis after 48 hours. Both EB doses activate membrane estrogen receptor-α complexed with metabotropic glutamate receptor-1a (mERα-mGluR1a), activating a multisynaptic circuit in the arcuate nucleus (ARH). I hypothesized that 50µg EB downregulates ERα and mERα-mGluR1a complexes in the ARH. Total ARH ERα protein was reduced 48 hours after 50µg EB, but the 2µg dose was intermediate between oil and 50µg EB. mERα that co-immunoprecipitated with mGluR1a were greater 48 hours after 2µg EB treatment versus rats receiving 50µg EB. Progesterone signals rapidly but not through progesterone receptor-dopamine D1 receptor complexes. These results indicate 2µg EB maintains but 50µg EB downregulates mERα-mGluR1a to regulate lordosis.

Indexing (document details)
Advisor: Sinchak, Kevin
Commitee: Tsai, Houng-Wei, Young, Kelly
School: California State University, Long Beach
Department: Biological Sciences
School Location: United States -- California
Source: MAI 52/03M(E), Masters Abstracts International
Subjects: Neurosciences, Endocrinology
Keywords: Estrogen receptor, Lordosis, Membrane signaling
Publication Number: 1524139
ISBN: 978-1-303-52117-1
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