Sexual receptivity in the female rat is dependent on dose and duration of estradiol exposure. A 2µg dose of estradiol benzoate (EB) primes reproductive behavior circuits but without subsequent progesterone does not facilitate lordosis. However, 50µg EB facilitates lordosis after 48 hours. Both EB doses activate membrane estrogen receptor-α complexed with metabotropic glutamate receptor-1a (mERα-mGluR1a), activating a multisynaptic circuit in the arcuate nucleus (ARH). I hypothesized that 50µg EB downregulates ERα and mERα-mGluR1a complexes in the ARH. Total ARH ERα protein was reduced 48 hours after 50µg EB, but the 2µg dose was intermediate between oil and 50µg EB. mERα that co-immunoprecipitated with mGluR1a were greater 48 hours after 2µg EB treatment versus rats receiving 50µg EB. Progesterone signals rapidly but not through progesterone receptor-dopamine D1 receptor complexes. These results indicate 2µg EB maintains but 50µg EB downregulates mERα-mGluR1a to regulate lordosis.
|Commitee:||Tsai, Houng-Wei, Young, Kelly|
|School:||California State University, Long Beach|
|School Location:||United States -- California|
|Source:||MAI 52/03M(E), Masters Abstracts International|
|Keywords:||Estrogen receptor, Lordosis, Membrane signaling|
Copyright in each Dissertation and Thesis is retained by the author. All Rights Reserved
The supplemental file or files you are about to download were provided to ProQuest by the author as part of a
dissertation or thesis. The supplemental files are provided "AS IS" without warranty. ProQuest is not responsible for the
content, format or impact on the supplemental file(s) on our system. in some cases, the file type may be unknown or
may be a .exe file. We recommend caution as you open such files.
Copyright of the original materials contained in the supplemental file is retained by the author and your access to the
supplemental files is subject to the ProQuest Terms and Conditions of use.
Depending on the size of the file(s) you are downloading, the system may take some time to download them. Please be