Injury to the avian hippocampus increases stem cell activity in the subventricular zone (SVZ) indicating possible self-repair. Radial glia in the SVZ act as stem cells that mitotically divide forming new cells. Some differentiate into neurons; others develop a glial phenotype. Experiment 1 revealed an immediate post-injury response through comparison of the number of new cells in an intact versus injured brain. Experiment 2 was a post-injury analysis over time. A peak in new cells was observed at 72 hours, but no significant effect of time (2, 72, or 168 hours) was found. Qualitative observations in Experiment 3 revealed a radial glial response within 2 hours of injury, with an early peak and steady decline thereafter. Reactive astrocytes appeared 26 hours post-lesion, and increased thereafter. This study was the first to develop the procedure in birds, successfully double-label newly born radial glia, and show self-renewal of the stem cell layer.
|Advisor:||Lee, Diane W.|
|Commitee:||Green, Kenneth, Zavala, Arturo|
|School:||California State University, Long Beach|
|School Location:||United States -- California|
|Source:||MAI 52/01M(E), Masters Abstracts International|
|Subjects:||Neurobiology, Experimental psychology, Physiological psychology|
|Keywords:||Hippocampus, Neurogenesis, Radial glia, Reactive astrocytes, Subventricular zone, Traumatic brain injury|
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